Recombinant Cynomolgus IL-17A / CTLA-8 Protein, His Tag, 100 µg

Background
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Source
Recombinant Cynomolgus IL-17A, His Tag (ILA-C5249) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (Accession # G7P4U9-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 18-19 kDa,24-25 kDa and 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "IL-17A promotes endothelial cell senescence by up-regulating the expression of FTO through activating JNK signal pathway"
Li, Luo, Zhang et al
Biogerontology (2022)
(2)  "Acinetobacter baumannii reinforces the pathogenesis by promoting IL-17 production in a mouse pneumonia model"
Zhou, Xiang, Wang et al
Med Microbiol Immunol (2022)
(3) "Cytokine profiling in patients with hepatic glycogen storage disease: Are there clues for unsolved aspects?"
Colonetti, Pinto E Vairo, Siebert et al
Cytokine (2022) 162, 156088
Showing 1-3 of 21357 papers.