Recombinant Mouse IL-17A / CTLA-8 Protein, His Tag (MALS verified), 100 µg

Background
Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.

Source
Recombinant Mouse IL-17A, His Tag (CT8-M5240) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Ala 158 (Accession # Q62386-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 15.8 kDa. The protein migrates as 17-19 kDa and 21-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Interleukin-17A knockout or self-recovery alleviated autoimmune reaction induced by fluoride in mouse testis"
Wu, Yang, Sun et al
Sci Total Environ (2023)
(2)  "Modified Gegen Qinlian decoction ameliorated ulcerative colitis by attenuating inflammation and oxidative stress and enhancing intestinal barrier function in vivo and in vitro"
Wang, Zhang, Zhang et al
J Ethnopharmacol (2023)
(3) "Dissecting the causal association between inflammation and post-traumatic stress disorder: A bidirectional Mendelian randomization study"
Zuo, Zhuang, Yang et al
J Affect Disord (2023)
Showing 1-3 of 22075 papers.