Recombinant Human M-CSF / CSF-1 Protein, His Tag, 50µg

Background
The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.

Source
Recombinant Human M-CSF, His Tag (MCF-H5247) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).
Predicted N-terminus: His 

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 26.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Development of Recombinant Oncolytic rVSV-mIL12-mGMCSF for Cancer Immunotherapy"
Ryapolova, Minskaia, Gasanov et al
Int J Mol Sci (2023) 25 (1)
(2) "Chitosan-Based Dressing as a Sustained Delivery System for Bioactive Cytokines"
Lewicki, Zwoliński, Hovagimyan et al
Int J Mol Sci (2023) 25 (1)
(3) "Biological Effects of "Inflammageing" on Human Oral Cells: Insights into a Potential Confounder of Age-Related Diseases"
Alexakou, Bakopoulou, Apatzidou et al
Int J Mol Sci (2023) 25 (1)
Showing 1-3 of 33033 papers.