Recombinant human 2B4 / CD244 / SLAMF4 Protein, His-Tag, 100µg

Background
Natural killer cell receptor 2B4 is also known as NK cell type I receptor protein 2B4 (NKR2B4 or h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4, CD antigen CD244. NKR2B4 / CD244 contains two Ig-like (immunoglobulin-like) domains. CD244 is expressed in spleen, PBL, followed by lung, liver, testis and small intestine. CD244 interacts with CD48. Following phosphorylation, CD244 is able to recruit PTPN11/SHP-2 and SH2D1A/SAP. SLAMF4 modulate other receptor-ligand interactions to enhance leukocyte activation. CD244/2B4 is the only heterophilic receptor of SLAM family.

Source
Recombinant Human 2B4, His Tag (2B4-H5224) is expressed from human 293 cells (HEK293). It contains AA Cys 22 - Arg 221 (Accession # Q9BZW8-2).
Predicted N-terminus: Cys 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.1 kDa. The protein migrates as 35-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Ferroptosis modulates invasion and migration in prostate cancer PC-3M subclones"
Rao, Liu, Ma et al
Exp Cell Res (2025) 454 (2), 114847
(2) "Profiling NK cell receptor repertoire of women with idiopathic recurrent pregnancy loss"
Gurbanova, Feyaerts, Benner et al
J Reprod Immunol (2025) 173, 104803
(3) "Aptamer Engineering: Strategies for Discovering Functional Nucleic Acids for Next-Generation Diagnostics and Biosensing"
Nguyen, Meziadi, Brunner et al
Adv Sci (Weinh) (2025)
Showing 1-3 of 5238 papers.