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Recombinant Mouse CD28 Protein, His Tag, active dimer (MALS verified), 100µg  

Recombinant Mouse CD28 Protein, His Tag, active dimer (MALS verified), 100µg

Recombinant Mouse CD28 Protein (Mouse CD28) Asn 20 - Lys 149, produced in human 293 cells (HEK293), His Tag, active dimer (MALS verified)

Synonym: Recombinant, Mouse, Protein,CD28,Tp44

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Availability: within 7 days

403,00 €

T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.

Recombinant Mouse CD28, His Tag (CD8-M52H6) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).
Predicted N-terminus: Asn 20

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 30-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Less than 1.0 EU per μg by the LAL method.

>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Please refer to product data sheet.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

See Certificate of Analysis for details of reconstitution instruction and specific concentration.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

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