Recombinant Mouse B7-H2 / ICOSLG Protein, Fc Tag, 100µg

Background
ICOS ligand (ICOSLG) is also known as B7 homolog 2 (B7-H2), B7-related protein 1 (B7RP-1) and CD antigen CD275, which belongs to the immunoglobulin superfamily and BTN/MOG family. ICOSLG contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. Isoform 1 is widely expressed, while isoform 2 is detected only in lymph nodes, leukocytes and spleen. B7-H2 is ligand for the T-cell-specific cell surface receptor ICOS. B7-H2 acts as a costimulatory signal for T-cell proliferation and cytokine secretion and induces also B-cell proliferation and differentiation into plasma cells. B7-H2 could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function.

Source
Recombinant Mouse B7-H2, Fc Tag (B72-M5258) is expressed from human 293 cells (HEK293). It contains AA Glu 47 - Lys 279 (Accession # Q9JHJ8-1).
Predicted N-terminus: Glu 47

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 52.7 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Role of B7 family members in glioma: Promising new targets for tumor immunotherapy"
Wang, Li, Wang et al
Front Oncol (2022) 12, 1091383
(2)  "Clinical significance and correlation of PD-L1, B7-H3, B7-H4, and TILs in pancreatic cancer"
Yang, Tian, Gao et al
BMC Cancer (2022) 22 (1), 584
(3) "B7-H4 Immune Checkpoint Protein Affects Viability and Targeted Therapy of Renal Cancer Cells"
Emaldi, Nunes-Xavier
Cells (2022) 11 (9)
Showing 1-3 of 69 papers.