Recombinant Rhesus macaque CD155 / PVR Protein, His Tag, 100µg

Background
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.

Source
Recombinant Rhesus macaque CD155, His Tag (CD5-R52H5) is expressed from human 293 cells (HEK293). It contains AA Asp 28 - Asn 343 (Accession # Q0MSE6-1). Predicted N-terminus: Asp 28

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.3 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "Enhancing T cell anti-tumor efficacy with a PD1-TIGIT chimeric immune-checkpoint switch receptor"
Zhao, Dong, Deng et al
Oncoimmunology (2023) 12 (1), 2265703
(2)  "Intensified NK cell therapy in combination with low-dose chemoradiotherapy against human colorectal cancer"
Nguyen, Bae, Park et al
Cancer Immunol Immunother (2023)
(3)  "Renal tubular epithelial cells are constitutive non-cognate stimulators of resident T cells"
Sadasivam, Jie, Hamad
Cell Rep (2023) 42 (10), 113210
Showing 1-3 of 520 papers.