Recombinant Mouse PD-L2 / B7-DC Protein, Fc Tag, 100µg

Background
Programmed cell death 1 ligand 2 (PD-L2 or PDCD1 ligand 2) is also known as Butyrophilin B7-DC, CD antigen CD273, which belongs to the immunoglobulin superfamily or BTN/MOG family. The expression of PD-L2 is up-regulated by IFNG/IFN-gamma stimulation in monocytes and induced on dendritic cells grown from peripheral blood mononuclear cells with CSF2 and IL-4. PD-L2 Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. PD-L2 interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.

Source
Recombinant Mouse PD-L2, Fc Tag (PD2-M5254) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Arg 219 (Accession # NP_067371).
Predicted N-terminus: Leu 20

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.2 kDa. As a result of glycosylation, the protein migrates as 60-70 kDa under reducing (R) condition, and 120-140 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Upregulation of PD-1 and its ligands and expansion of T peripheral helper cells in the nephritic kidneys of lupus-prone BXSB-Yaa mice"
Moriyama, Katsumata, Okamoto et al
Lupus (2024)
(2) "Increased co-expression of ICOS and PD-1 predicts poor overall survival in patients with acute myeloid leukemia"
Pan, Cai, Wei et al
Immunobiology (2024) 229 (3), 152804
(3) "Expression and prognostic significance of the PD-1/PD-L1 pathway in AIDS-related non-Hodgkin lymphoma"
Zhao, Cai, Xiao et al
Cancer Med (2024) 13 (7), e7195
Showing 1-3 of 1592 papers.

Citations
"Quantitative phosphoproteomic analysis reveals involvement of PD-1 in multiple T cell functions"
Authors: Tocheva AS, Peled M, Strazza M, et al.
Journal: The Journal of biological chemistry 2020
Application: ELISA