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Background
Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.
Source
Recombinant Rabbit CD47, Fc Tag (CD7-R5257) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Glu 139 (Accession # G1SKT7).
Predicted N-terminus: Gln 19
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 40.4 kDa. As a result of glycosylation, the protein migrates as 48-58 kDa under reducing (R) condition, and 85-110 kDa and 160-220 kDa under non-reducing (NR) condition (SDS-PAGE)
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Bioactivity
Please refer to poduct data sheet.
(1) "Proteomic study of vitreous in proliferative diabetic retinopathy patients after treatment with aflibercept: a quantitative analysis based on 4D label-free technique"
Feng, Gao, Liang et al
Int J Ophthalmol (2024) 17 (4), 676-685
(2) "Spatial distribution of tumor-resident macrophages as predictive biomarkers in endometrial cancer"
Matsukawa, Yoshikawa, Liu et al
J Obstet Gynaecol Res (2024)
(3) "Difference between Keratinized- and Non-Keratinized-Originating Epithelium in the Process of Immune Escape of Oral Squamous Cell Carcinoma"
Kitsukawa, Fukumoto, Hyodo et al
Int J Mol Sci (2024) 25 (7)
Showing 1-3 of 2707 papers.
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