Recombinant Cynomolgus SIRP alpha / CD172a Protein, His Tag (MALS verified), 100 µg

Background
Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.

Source
Recombinant Cynomolgus SIRP alpha, His Tag (SIA-C52H7) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 370 (Accession # NP_001271679.1).
Predicted N-terminus: Glu 31

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.0 kDa. The protein migrates as 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Immunoregulatory function of SP-A"
Dong, Pang, Li et al
Mol Immunol (2024) 166, 58-64
(2) "A new role for erythropoietin in the homeostasis of red blood cells"
Arias, Valente-Leal, Bertocchini et al
Commun Biol (2024) 7 (1), 58
(3) "Preclinical evaluation of the efficacy of an antibody to human SIRPα for cancer immunotherapy in humanized mouse models"
Saito, Iida-Norita, Afroj et al
Front Immunol (2023) 14, 1294814
Showing 1-3 of 160 papers.

Citations

(1) "Novel SIRPα Antibodies That Induce Single-Agent Phagocytosis of Tumor Cells while Preserving T Cells"
Authors: Andrejeva G, Capoccia BJ, Hiebsch RR, et al.
Journal: J Immunol 2021
Application: ELISA