Recombinant Biotinylated Human SIRP gamma / CD172g Protein, His,Avitag™ (MALS verified), 25 µg

Background
Signal-regulatory protein gamma (SIRPG or SIRP-gamma) is also known as CD172 antigen-like family member B (CD172g), Signal-regulatory protein beta-2 (SIRPB2), is a single-pass type I membrane protein. SIRPG is expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells. SIRPG interacts with CD47. On binding with CD47, CD172g mediates cell-cell adhesion.

Source
Recombinant Biotinylated Human SIRP gamma, His,Avitag (SIG-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 29 - Ser 364 (Accession # AAH64532.1).
Predicted N-terminus: Glu 29

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 40.7 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "SIRPγ-CD47 Interaction Positively Regulates the Activation of Human T Cells in Situation of Chronic Stimulation"
Dehmani, Nerrière-Daguin, Néel et al
Front Immunol (2021) 12, 732530
(2) "Nanocages displaying SIRP gamma clusters combined with prophagocytic stimulus of phagocytes potentiate anti-tumor immunity"
Choi, Nam, Kim et al
Cancer Gene Ther (2021) 28 (9), 960-970
(3) "A Novel Gamma Distributed Random Variable (RV) Generation Method for Clutter Simulation with Non-Integral Shape Parameters"
Chen, Luo, Hu
Sensors (Basel) (2020) 20 (4)
Showing 1-3 of 21 papers.