Recombinant Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag, low endotoxin (active trimer) (MALS verified), 100 µg

Background
Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Source
Recombinant Human 4-1BB Ligand (71-254), Fc Tag, premium grade (41L-H5269) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).
Predicted N-terminus: Pro
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 87.5 kDa. The protein migrates as 150-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.01 EU per μg by the LAL method.

Protein A
<5 ppm of protein tested by ELISA.

Host Cell Protein
<0.5 ng/µg of protein tested by ELISA.

Host Cell DNA
<0.02 ng/μg of protein tested by qPCR.

Sterility
The sterility testing was performed by membrane filtration method.

Mycoplasma
Negative.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 24 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Preclinical characterization and phase 1 results of ADG106 in patients with advanced solid tumors and non-Hodgkin's lymphoma"
Ma, Luo, Zhang et al
Cell Rep Med (2024)
(2) "Natural killer cells drive 4-1BBL positive uveal melanoma towards EMT and metastatic disease"
Neo, Oliveira, Tong et al
J Exp Clin Cancer Res (2024) 43 (1), 13
(3) "M9657 Is a Bispecific Tumor-Targeted Anti-CD137 Agonist That Induces MSLN-Dependent Antitumor Immunity without Liver Inflammation"
Xu, Zhou, Webb et al
Cancer Immunol Res (2024) 12 (2), 195-213
Showing 1-3 of 741 papers.