Recombinant Cynomolgus / Rhesus macaque OX40 Ligand / TNFSF4 Protein, Mouse IgG2a Fc Tag, 100 µg

Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

Source
Recombinant Cynomolgus / Rhesus macaque OX40 Ligand, Mouse IgG2a Fc Tag (OXL-R5259) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # F7FL80-1). In the region Gln 51 - Leu 183, the AA sequence of Cynomolgus and Rhesus macaque OX40 Ligand are homologus.
Predicted N-terminus: Glu

Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 42.5 kDa. The protein migrates as 47-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "The prognostic value of a combined immune score in tumor and immune cells assessed by immunohistochemistry in triple-negative breast cancer"
Choi, Lee, Jin et al
Breast Cancer Res (2023) 25 (1), 134
(2)  "Construction and validation of a immune-related prognostic gene DHRS1 in hepatocellular carcinoma based on bioinformatic analysis"
Xu, Wang, Meng et al
Medicine (Baltimore) (2023) 102 (42), e35268
(3) "Ferroptosis-related lncRNAs guiding osteosarcoma prognosis and immune microenvironment"
Yang, Su, Xu et al
J Orthop Surg Res (2023) 18 (1), 787
Showing 1-3 of 954 papers.