Recombinant, human, Glycogen synthase kinase-3 beta (GSK3-beta), protein kinase domain, GST-HIS6 Tag, 10 µg

Background: GSK3 beta is a serine/threonine protein kinase and works as regulatory kinase for its namesake, Glycogen synthase. It acts also as a downstream regulatory switch for numerous signaling pathways, including cellular responses to WNT, Growth Factors, Insulin, RTK (Receptor Tyrosine Kinases), Hedgehog pathways, and GPCR (G-Protein-Coupled Receptors) in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules.

recombinant human GSK3-beta (Glycogen Synthase Kinase 3 beta), amino acids M1-T420 (protein kinase domain), recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 76.312 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris/HCl pH 8.0, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.276 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 6300 pmol/mg x min

Entrez Gene ID: 2932
UniProtKB: P49841

Ordering information: shipped on dry ice

Product specific literature:

Sutherland C., Leighton I.A., Cohen P. (1993) "Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling." Biochem. J. 296:15-19

Ali A., Hoeflich K.P., Woodgett J.R.(2001) "Glycogen synthase kinase-3: properties, functions, and regulation." Chem. Rev. 101:2527-2540

Product Citations:

Prinz B, Harvey KL, Wilcke L, Ruch U, Engelberg K, Biller L, Lucet I, Erkelenz S, Heincke D, Spielmann T, Doerig C, Kunick C, Crabb BS^, Gilson PR, Gilberger TW^, (2016) "Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites." Sci Rep. 2016 Oct 4;6:34479.