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Recombinant, human, Glycogen synthase kinase-3 beta (GSK3-beta), protein kinase domain, GST-HIS6 Tag, 10 µg  

Recombinant, human, Glycogen synthase kinase-3 beta (GSK3-beta), protein kinase domain, GST-HIS6 Tag, 10 µg

Recombinant human Glycogen synthase kinase-3 beta (GSK3-beta), protein kinase domain, amino acids M1-T420, active enzyme

Alternate names: GSK-3-beta, glycogen synthase kinase 3 beta, Serine/threonine-protein kinase GSK3B, GSK3 beta kinase

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Availability: within 14 days

450,00 €

Background: GSK3 beta is a serine/threonine protein kinase and works as regulatory kinase for its namesake, Glycogen synthase. It acts also as a downstream regulatory switch for numerous signaling pathways, including cellular responses to WNT, Growth Factors, Insulin, RTK (Receptor Tyrosine Kinases), Hedgehog pathways, and GPCR (G-Protein-Coupled Receptors) in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules.

recombinant human GSK3-beta (Glycogen Synthase Kinase 3 beta), amino acids M1-T420 (protein kinase domain), recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 76.312 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM Tris/HCl pH 8.0, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.276 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 6300 pmol/mg x min

Entrez Gene ID: 2932
UniProtKB: P49841

Ordering information: shipped on dry ice

Product specific literature:

Sutherland C., Leighton I.A., Cohen P. (1993) "Inactivation of glycogen synthase kinase-3 beta by phosphorylation: new kinase connections in insulin and growth-factor signalling." Biochem. J. 296:15-19

Ali A., Hoeflich K.P., Woodgett J.R.(2001) "Glycogen synthase kinase-3: properties, functions, and regulation." Chem. Rev. 101:2527-2540

Product Citations:

Prinz B, Harvey KL, Wilcke L, Ruch U, Engelberg K, Biller L, Lucet I, Erkelenz S, Heincke D, Spielmann T, Doerig C, Kunick C, Crabb BS, Gilson PR, Gilberger TW, (2016) "Hierarchical phosphorylation of apical membrane antigen 1 is required for efficient red blood cell invasion by malaria parasites." Sci Rep. 2016 Oct 4;6:34479.

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