Recombinant human Mammalian STE20-like protein kinase 2 (MST2), 10 µg

Background: Mammalian Sterile20-like kinase 2 (Mst2) is a serine/threonine kinase which represents a member of the germinal center kinases (GCKs), a family of ‘Sterile 20 (STE20) like kinases’. MST1 and MST2 are mammalian homologs of drosophila ‘Hpo’ playing a fundamental role in organ size control and tumor suppression. Both proteins share high sequence homology with identical N-terminal kinase domains and C-terminal SARAH (Salvador, RASSF and Hpo homology) domains, that mediates hetero- and homo-dimerisation. MST1/2 in conjunction with an adaptor protein Salvador phosphorylates and activates LATS1/2 kinases, finally results in an inhibition of cell proliferation and promotion of apoptosis. Furthermore, MST1/2 kinases have been implicated in immune regulation including T cell proliferation, activation, and homeostasis, lymphocyte chemotaxis and trafficking, as well as inflammatory responses.

Human MST2 /STEK3 (mammalian sterile 20-like 2, Serine/threonine-protein kinase 3), untagged protein, recombinant and active enzyme, full lenght (amino acids M1-F491), untagged, expressed in Sf9 insect cells

Theoretical MW : 56.884 kDa (fusion proteins)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography followed by 3C mediated removal of the GST tag
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.271 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity : 47,000 pmol/mg min

Entrez Gene ID: 6788
UniProtKB: Q13188

Ordering information: shipped on dry ice

Product specific literature:

Avruch J1, Zhou D, Fitamant J, Bardeesy N. (2011) ”Mst1/2 signalling to Yap: gatekeeper for liver size and tumour development.” Br J Cancer. ;104(1):24-32.

Yin H1, Shi Z, Jiao S, Chen C, Wang W, Greene MI, Zhou Z. (2012) “Germinal center kinases in immune regulation.” Cell Mol Immunol. (6):439-45.

de Souza PM1, Lindsay MA. (2004) „Mammalian Sterile20-like kinase 1 and the regulation of apoptosis.” Biochem Soc Trans. (Pt3):485-8.

Liu G1, Shi Z2, Jiao S2, Zhang Z1, Wang W2, Chen C2, Hao Q, Zhang M2, Feng M2, Xu L2, Zhang Z2, Zhou Z3, Zhang M4. (2014) Structure of MST2 SARAH domain provides insights into its interaction with RAPL.J Struct Biol.185(3):366-74.