Recombinant human biotinylated ERK2 (p42, MAPK1), protein kinase, unactive, 5 µg

Background: Extracellular signal-regulated kinase (ERK) is a serine/threonine protein kinase that functions as the major effector of the Ras protein, a small GTPase. The so called “ERK cascade” consists of three enzymes, the initial GTPase-regulated kinase Raf, (MAP3K) that phosphorylates and activates an intermediate kinase MEK (MAP2K) that, in turn, phosphorylates a threonine and a tyrosine residue of the activation loop of the effector kinase ERK (MAPK).  Both, ERK1 and ERK2 share a high sequence identity (90%) and are co-expressed in most tissues but differ in their relative abundance. The substrate specificities of the  ERK1 and ERK2 protein kinases are very similar. The consensus sequence for ERK1 substrates has been identified as -Pro- Leu-Ser/Thr-Pro. Both kinases are involved in proliferation, differentiation, cell cycle processes, and survival.  ERK1 / 2 differentially phosphorylates a variety of nuclear (Elk-1, c-Myc), cytosolic (MNK1/2, Raf) and cytoskeletal (MAP2, Tau) targets. Since the ERK pathway is often up-regulated in human tumors it represents an attractive target for anticancer drugs development.

Biotinylated protein is not phosphorylated and activated by MKKs. Suitable as substrate for kinase activity assays and for western blot analyses.

Theoretical MW:  42.8 kDa (fusion proteins)
Expression system:  E.coli
Purification:   Immobilized metal affinity chromatography (IMAC), chemically biotinylated, gel filtration
Storage buffer:  20 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 20% glycerol, pH 8.0
Purity:  >95 %
Protein concentration: 0,27 mg/ml (Bradford method using BSA as standard protein)

Recombinant human biotinylated Extracellular signal-regulated kinase2 (ERK2) / Mitogen-activated protein kinase 1 (MAPK1), unactive enzyme. Biotinylated protein is not phosphorylated and activated by MKKs. Suitable for kinase molecule interaction analyses using SPR, as substrate for kinase activity assays and for western blot analyses.

Entrez Gene ID:5594
UniProtKB: P28482

Ordering information: shipped on dry ice

Product specific literature references:

Gonzalez FA, Raden DL, Davis RJ (1991) "Identification of substrate recognition determinants for human ERK1 and ERK2 protein kinases" J. Biol. Chem. 266(33):22159-63

Meloche S, Pages G, Pouyssegur J (1992) "Functional expression and growth factor activation of an epitope-tagged p44 mitogen-activated protein kinase, p44mapk" Mol Biol Cell 3(1):63-71

Hartsough MT, Mulder KM (1995) "Transforming growth factor beta activation of p44mapk in proliferating cultures of epithelial cells" J Biol Chem. 270(13):7117-24

Songyang Z, Lu KP, Cantley LC et al. (1996) "A structural basis for substrate specificities of protein Ser/Thr kinases: primary sequence preference of casein kinases I and II, NIMA, phosphorylase kinase, calmodulin-dependent kinase II, CDK5, and Erk1" Mol Cell Biol. 16(11):6486-93

Wortzel I, Seger R. (2011) “The ERK Cascade: Distinct Functions within Various Subcellular Organelles.” Genes Cancer. 2(3):195-209.

Kohno M, Pouyssegur J(2006).” Targeting the ERK signaling pathway in cancer therapy”. Ann Med.;38(3):200-11.

Mazzucchelli C, Vantaggiato C, Brambilla R et al. (2002) "Knockout of ERK1 MAP kinase enhances synaptic plasticity in the striatum and facilitates striatal-mediated learning and memory" Neuron 34(5):807-20