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Recombinant human JNK2 / MAPK9, protein kinase, 40 µg  

Recombinant human JNK2 / MAPK9, protein kinase, 40 µg

Recombinant human, c-Jun N-terminal kinase 2 (JNK2), active enzyme

Alternate names: recombinant, human, SAPK,  MAPK-9, JNK2 beta, JNK2B, Stress-activated protein kinase 1a, Mitogen-activated protein kinase 9, c-Jun N-terminal kinase 2

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255,00 €

Background: The c-Jun N-terminal kinases JNK / SAPK are members of the MAP kinase family acting as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. Complete activation of JNKs requires dual phosphorylation within the activation loop by MKK4 and / or MKK7. Activated JNK2 can in turn phosphorylate transcription factors such as c-Jun and ATF2, which participate in the activation and formation of the AP-1 complex and anti-apoptotic Bcl-2 family members as well as pro-apoptotic BAD proteins. In contrast, the inactive JNKs have been shown to cause degradation of several transcription factors (c-Jun, ATF2, p53). The expression level and the activity of JNKs is altered in various cancer types. Based on several experiments inhibition of JNK2 is associated with an reduced tumor growth, whereas blocking of JNK1 seems to results in an increased risk of tumorigenesis

Human JNK2a/SAPK1a, recombinantly expressed in E. coli. Autophosphorylation < 5%. No protease activity detectable.

Expression system: E.coli
Purification: one step purification using Ni-NTA-agarose
Purity: >95% by SDS-PAGE
Storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 50 % glycerol, pH 8.5
Storage: -80°C
Protein Concentration: 1.3 mg/ml
Specific activity: 600 u/mg (1 Unit is defined as 1 picomole phosphate transferred to c-jun (1-169-N-His-tag) per min at 30 °C)

EntrezGene ID: 5601
UniProtKB: P45984

Ordering information: shipped on dry ice

Product specific literature references:

Sluss HK, Barrett T, Derijard B, Davis RJ (1994) "Signal transduction by tumor necrosis factor mediated by JNK protein kinases" Mol Cell Biol. 14(12):8376-84

Kallunki T, Su B, Tsigelny I, Sluss HK, Derijard B, Moore G, Davis R, Karin M (1994) "JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation" Genes Dev. 8(24):2996-3007

Uciechowski P, Saklatvala J, von der Ohe J, Resch K, Szamel M, Kracht M (1996) "Interleukin 1 activates jun N-terminal kinases JNK1 and JNK2 but not extracellular regulated MAP kinase (ERK) in human glomerular mesangial cells" FEBS Lett. 394(3):273-8

Yang DD, Conze D, Whitmarsh AJ, Barrett T, Davis RJ, Rincon M, Flavell RA (1998) "Differentiation of CD4+ T cells to Th1 cells requires MAP kinase JNK2" Immunity 9(4):575-85

Sabapathy K, Hu Y, Kallunki T, Schreiber M, David JP, Jochum W, Wagner EF, Karin M (1999) "JNK2 is required for efficient T-cell activation and apoptosis but not for normal lymphocyte development" Curr Biol. 9(3):116-25

Kuan CY, Yang DD, Samanta Roy DR, Davis RJ, Rakic P, Flavell RA (1999) "The Jnk1 and Jnk2 protein kinases are required for regional specific apoptosis during early brain development" Neuron. 22(4):667-76

Ham J, Eilers A, Whitfield J, Neame SJ, Shah B (2000) "c-Jun and the transcriptional control of neuronal apoptosis" Biochem. Pharmacol. 60(8):1015-21

Hochedlinger K, Wagner EF, Sabapathy K (2002) "Differential effects of JNK1 and JNK2 on signal specific induction of apoptosis" Oncogene 21(15):2441-5

Arbour N, Naniche D, Homann D, Davis RJ, Flavell RA, Oldstone MB (2002) "c-Jun NH(2)-terminal kinase (JNK)1 and JNK2 signaling pathways have divergent roles in CD8(+) T cell-mediated antiviral immunity" J Exp Med. 195(7):801-10

Bogoyevitch MA, Boehm I, Oakley A, Ketterman AJ, Barr RK (2004) "Targeting the JNK MAPK cascade for inhibition: basic science and therapeutic potential" Biochim Biophys Acta. 1697(1-2):89-101

Sabapathy K, Hochedlinger K, Nam SY, Bauer A, Karin M, Wagner EF (2004) "Distinct roles for JNK1 and JNK2 in regulating JNK activity and c-Jun-dependent cell proliferation" Mol Cell. 15(6):843-4

Tafolla E, Wang S, Wong B, Leong J, Kapila YL (2005) "JNK1 and JNK2 Oppositely Regulate p53 in Signaling Linked to Apoptosis Triggered by an Altered Fibronectin Matrix: JNK LINKS FAK AND p53" J Biol Chem. 280(20):19992-9

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