Recombinant Human Alpha-Synuclein Protein, His Tag, 200 µg

Background
Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

Source
Recombinant Human Alpha-Synuclein, His Tag (ALN-H52H8) is expressed from E.coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Sterility
The sterility testing was performed by membrane filtration method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Exploring Parkinson-associated Kinases for CRISPR/Cas9-Based Gene Editing: Beyond Alpha-Synuclein
Mansour, El-Khatib"
Ageing Res Rev (2023)
(2) "Brassisterol A, a new ergosterol from co-cultivation of fungi attenuates neuroinflammation via targeting NLRP3/caspase-1/GSDMD pathway"
Tong, Kong, Zhang et al
Bioorg Chem (2023) 142, 106955
(3) "Synaptotagmin-11 regulates immune functions of microglia in vivo"
Zhang, Yang, Li et al
J Neurochem (2023)
Showing 1-3 of 16977 papers.