Recombinant Human Iba1 / AIF-1 Protein, His Tag, 100 µg

Background
Allograft inflammatory factor 1 (AIF1), also known as IBA1, daintain and Protein G1, is an actin-binding protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Source
Recombinant Human Iba1, His Tag (IB1-H51H9) is expressed from E.coli cells. It contains AA Met 1 - Pro 147 (Accession # P55008-1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

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Qin, Huang, Liao et al
J Mol Histol (2024)
(2) "Intravenous administration of anti-CD47 antibody augments hematoma clearance, mitigates acute neuropathology, and improves cognitive function in a rat model of penetrating traumatic brain injury"
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J Neurotrauma (2024)
(3) "Probiotics and microbial metabolites maintain barrier and neuromuscular functions and clean protein aggregation to delay disease progression in TDP43 mutation mice"
Zhang, Xia, Sun
Gut Microbes (2024) 16 (1), 2363880
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