Recombinant Human Iba1 / AIF-1 Protein, His Tag, 100 µg

Background
Allograft inflammatory factor 1 (AIF1), also known as IBA1, daintain and Protein G1, is an actin-binding protein. Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Source
Recombinant Human Iba1, His Tag (IB1-H51H9) is expressed from E.coli cells. It contains AA Met 1 - Pro 147 (Accession # P55008-1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 18.6 kDa. The protein migrates as 18-20 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Neuronal melatonin type 1 receptor overexpression promotes M2 microglia polarization in cerebral ischemia/reperfusion-induced injury"
Suofu, Jauhari, Nirmala et al
Neurosci Lett (2022)
(2) "Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis"
Wan, Eguchi, Sakamoto et al
Brain Behav Immun Health (2023) 27, 100573
(3) "Precise measurement of gene expression changes in mouse brain areas denervated by injury"
Schlaudraff, Paul, Deller et al
Sci Rep (2022) 12 (1), 22530
Showing 1-3 of 4340 papers.