Recombinant Cynomolgus Axl Protein, His Tag, 100 µg

Background
AXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.

Source
Recombinant Cynomolgus Axl, His Tag (AXL-C52H3) is expressed from human 293 cells (HEK293). It contains AA Pro 27 - Pro 293 (Accession # XP_005589384.2).
Predicted N-terminus: Pro 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.7 kDa. The protein migrates as 42-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Axl receptor induces efferocytosis, dampens M1 macrophage responses and promotes heart pathology in Trypanosoma cruzi infection"
Rigoni, Vellozo, Guimarães-Pinto et al
Commun Biol (2022) 5 (1), 1421
(2) "Investigation of Causal Effects of Protein Biomarkers on Cardiovascular Disease in Persons with HIV"
Reilly, Borges, Baker et al
J Infect Dis (2022)
(3) "GAS6/Axl is associated with AMPK activation and attenuates H2O2-induced oxidative stress"
Liang, Yang, Wu et al
Apoptosis (2022)
Showing 1-3 of 2416 papers.