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Recombinant Biotinylated Human TGF-beta RII / TGFBR2 Protein, His,Avitag™, 25µg  

Recombinant Biotinylated Human TGF-beta RII / TGFBR2 Protein, His,Avitag™, 25µg

Recombinant Biotinylated Human TGF-beta RII / TGFBR2 Protein,  AA Thr 23 - Asp 159, expressed in human HEK293 cells, His-Tag, Avitag™

Synonyms: TGFBR2, TGFR2, TbetaR-II, TGFβR2, Transforming growth factor-beta 2

More details

TG2-H82E4-25

Availability: within 7 days

420,00 €

Background: TGF-beta receptor type-2 (TGFBR2 or TGFR-2) is also known as TGF-beta type II receptor, Transforming growth factor-beta receptor type II, TbetaR-II, TGFβR2, which is a homodimer or heterohexamer, belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family and TGFB receptor subfamily. TGFR2 / TGFBR2 binds TGF-β1 / TGFB1 and TGF-β3 / TGFB3 with high affinity and TGF-β2 / TGFB2 with a much lower affinity. This type I I receptor forms a heterodimeric complex with type I receptor and is essential for signal transduction. Upon ligand binding, the TGFR2 autophosphorylates its cytoplasmic domain and subsequently phosphorylates the downstream molecules which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation.

Source
Recombinant Biotinylated Human TGF-beta RII, His,Avitag (TG2-H82E4) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Asp 159 (Accession # P37173-1). Predicted N-terminus: Thr 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 19.1 kDa. The protein migrates as 28-40 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution

Bioactivity
Please refer to product data sheet.

References

(1) Wieser R., et al., 1995, EMBO J. 14:2199-2208.
(2) Deep S., et al., 2003, Biochemistry 42:10126-10139.
(3) Groppe J., et al., 2008, Mol. Cell 29:157-168.