Fluorescein labeled PKC substrate, 10 mg

The peptide KRPSKRAKA is N-terminal labeled with fluorescein isothiocyanate (FITC). It is a specific substrate for PKCα in in vitro kinase assays. The fluorescently labeled product is suitable for microfluidic mobility shift analyses (Caliper).

Appearance: Lyophilized solid (hygroscopic), Molecular Weight: 1543.83 g/mol, Purity: >95 % (HPLC, MS)

Product specific literature references:

Jill Coffin, Maria Latev, Xiahui Bi and Theo T. Nikiforov (2000) "Detection of Phosphopeptides by Fluorescence Polarization in the Presence of Cationic Polyamino Acids: Application to Kinase Assays " Anal Biochem. 15; 278(2):206-12.

Kwon YG, Mendelow M, Lawrence DS.(1994) "The active site substrate specificity of protein kinase C." J Biol Chem. 18;269(7):4839-44

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.