CDK5 substrate, 5 mg

The synthetic peptide PKTPKKAKKL is derived from the histone H1 and can be used as a substrate for cyclin-dependent kinase 5 (Cdk5) in in vitro kinase assays.It is phosphorylated by TPK II/cdk5 with a Km of 4.5µM.

Purity: 90 - 95 % (by HPLC, high quality, HQ)

Molecular weight 1138.7 g/mol

Form: lyophilized solid

Product specific literature references:

Beaudette KN, Lew J, Wang JH (1993) Substrate specificity characterization of a cdc2-like protein kinase purified from bovine brain. J Biol Chem 268: 20825-30

Qi Z, Huang QQ, Lee KY, Lew J, Wang JH (1995) "Reconstitution of neuronal Cdc2-like kinase from bacteria-expressed Cdk5 and an active fragment of the brain-specific activator. Kinase activation in the absence of Cdk5 phosphorylation" J. Biol. Chem. 270(18):10847-54

Sharma P, Steinbach PJ, Sharma M, Amin ND, Barchi JJ Jr, Pant HC (1999) "Identification of substrate binding site of cyclin-dependent kinase 5" J. Biol. Chem. 274(14):9600-6

Clare PM, Poorman RA, Kelley LC, Watenpaugh KD, Bannow CA, Leach KL (2001) "The cyclin-dependent kinases cdk2 and cdk5 act by a random, anticooperative kinetic mechanism" J. Biol. Chem. 276(51):48292-9

Evans DB1, Rank KB, Sharma SK. (2002) “A scintillation proximity assay for studying inhibitors of human tau protein kinase II/cdk5 using a 96-well format.” J Biochem Biophys Methods. 2002;50(2-3):151-61.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.