PKI, 100 µg

Background: The heat-stable protein kinase inhibitor PKI is a key role player for the regulation of PKA activity and for localizing the catalytic subunit of PKA in the cell, also working as a nuclear scavenger. This relatively small protein (77 amino acids) is extremely selective for PKA and  binds with very high affinity to the catalytic subunit of PKA (Ki = 0.5nM). It favors the nuclear export of the catalytic subunit thereby blocking the cAMP response element regulated gene expression. The recombinant heat-stable protein kinase inhibitor PKI supplied by Biaffin is a 8 kDa monomeric protein without fusion tag suitable for inhibition studies with PKA catalytic subunit. The amino acid sequence of the rabbit PKI shows 100% identity to the human PKI protein.

Purity: >95% (SDS-PAGE)
Storage buffer: 20mM MOPS (pH 7.0), 150 mM NaCl, 50% glycerol
Accession No.: P61926 (rabbit), identical to NP_006814 (human)

Ordering information: shipped on dry ice

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers. 

Product specific literature references:

Thomas J, Van Patten SM, Maurer RA et al. (1991) "Expression in Escherichia coli and characterization of the heat-stable inhibitor of the cAMP-dependent protein kinase" J Biol Chem. 266(17):10906-11

Olsen SR, Uhler MD (1991) "Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor" Mol Endocrinol. 5(9):1246-56

Fantozzi DA, Taylor SS, Meinkoth JL et al. (1992) "Effect of the thermostable protein kinase inhibitor on intracellular localization of the catalytic subunit of cAMP-dependent protein kinase" J Biol Chem. 267(24):16824-8

Wen W, Harootunian AT, Taylor SS et al. (1994) "Heat-stable inhibitors of cAMP-dependent protein kinase carry a nuclear export signal" J Biol Chem. 269(51):32214-20

Fantozzi DA, Harootunian AT, Meinkoth JL et al. (1994) "Thermostable inhibitor of cAMP-dependent protein kinase enhances the rate of export of the kinase catalytic subunit from the nucleus" J Biol Chem. 269(4):2676-86

Wen W, Meinkoth JL, Tsien RY, Taylor SS (1995) "Identification of a signal for rapid export of proteins from the nucleus" Cell 82(3):463-73

Wiley JC, Wailes LA, Idzerda RL, McKnight GS (1999) "Role of regulatory subunits and protein kinase inhibitor (PKI) in determining nuclear localization and activity of the catalytic subunit of protein kinase A" J Biol Chem. 274(10):6381-7

Peer - Reviewed Citations

Gressner OA1, Lahme B, Rehbein K, Siluschek M, Weiskirchen R, Gressner AM.(2008) "Pharmacological application of caffeine inhibits TGF-beta-stimulated connective tissue growth factor expression in hepatocytes via PPARgamma and SMAD2/3-dependent pathways."J Hepatol. 49(5):758-67.

 Kong D.¹, Jung S., Jeon H., Kim WJ., Kim YM., Ha KS. (2015) "A peptide array-based serological protein kinase A activity assay and its application in cancer diagnosis."Analyst. 140(19):6588-94.