Recombinant human mitogen-activated protein kinase kinase 1 (MEK1), 10 µg

Background: MEK1 or MAPKK 1 is a dual specificity protein kinase which acts as an essential component of the Mitogen-activated protein (MAP) kinases signal transduction pathway mediating diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. Upon interaction of extracellular ligands such as growth factors or cytokines to their cell-surface receptors activated RAS initiates RAF1 activation and in turn the activation of dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both kinases function specifically in the MAPK/ERK cascade, and catalyze the phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2.

Human recombinant mitogen activated protein kinase kinase 1 (MEK1, MKK1, MAPKK1, MAP2K1)i. Highly active and purified form which is activated by inserting two amino acid exchanges (S218D, S222D). Suitable for phosphorylation of MEK1 substrates and activation of ERK1 and ERK2.

Expression system: E.coli   
Molecular weight: 69.7 kDa
Protein concentration: 0,5 mg/ml (Bradford with BSA as standard)
Storage buffer: 50 mM Tris-HCl, 150 mM NaCl, 0.1 mM EDGT, 1 mM DTT, 270 mM sucrose,  pH 7.5
Specific activity: 543,000 U*/mg (* 1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide (RPRAATF) per min at 30 °C)
Purity: > 80% by SDS PAGE

Entrez Gene ID: 5604
UniProtKB: Q02750

Ordering information: shipped on dry ice

Product specific literature references:

Huang W, Kessler DS, Erikson RL (1995) "Biochemical and biological analysis of Mek1 phosphorylation site mutants" Mol Biol Cell. 6(3):237-45

Alessandrini A, Greulich H, Huang W, Erikson RL (1996) "Mek1 phosphorylation site mutants activate Raf-1 in NIH 3T3 cells" J Biol Chem. 271(49):31612-8

Schramek H, Feifel E, Healy E, Pollack V. (1997) "Constitutively active mutant of the mitogen-activated protein kinase kinase MEK1 induces epithelial dedifferentiation and growth inhibition in madin-darby canine kidney-C7 cells" J Biol Chem. 272(17):11426-33

Cheng M, Sexl V, Sherr CJ, Roussel MF (1998) "Assembly of cyclin D-dependent kinase and titration of p27Kip1 regulated by mitogen-activated protein kinase kinase (MEK1)" Proc Natl Acad Sci U S A. 95(3):1091-6

Alessandrini A, Namura S, Moskowitz MA, Bonventre JV (1999) "MEK1 protein kinase inhibition protects against damage resulting from focal cerebral ischemia" Proc Natl Acad Sci U S A 96(22):12866-9

Giroux S, Tremblay M, Charron J et al. (1999) "Embryonic death of Mek1-deficient mice reveals a role for this kinase in angiogenesis in the labyrinthine region of the placenta" Curr Biol. 9(7):369-72

Allen LF, Sebolt-Leopold J, Meyer MB (2003) "CI-1040 (PD184352), a targeted signal transduction inhibitor of MEK (MAPKK)" Semin Oncol. 30(5 Suppl 16):105-16