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Recombinant Mouse KITLG /SCF Protein, 50µg  

Recombinant Mouse KITLG /SCF Protein, 50µg

Recombinant Mouse KITLG /SCF Protein (Mouse SCF) Lys 26 - Ala 189, produced in human 293 cells (HEK293), His tag

Synonym: recombinant, mouse, protein, KITLG, FPH2, KL-1, Kitl, MGF, SCF, SF, SHEP7, KL

More details

SCF-M5228-050

Availability: within 7 days

456,00 €

Background
Kit ligand (KITLG) also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

Source
Recombinant Mouse KITLG /SCF Protein (Mouse SCF) Lys 26 - Ala 189 (Accession # NP_038626) was produced in human 293 cells (HEK293).

Molecular Characterization
Mouse SCF, fused with 6×His tag at the C-terminus, has a calculated MW of 19.1 kDa. The predicted N-terminus is Lys 26. DTT-reduced Protein migrates as 19-30 kDa due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse SCF by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5 - 8 ng/ml.

References

(1) Lu H.S., et al., 1992, Arch. Biochem. Biophys. 298:150-158.
(2) Wang Z.-Q., et al., 2009, Am. J. Hum. Genet. 84:672-677.
(3) Lev S., et al., 1992, J. Biol. Chem. (UNITED STATES) 267 (15): 10866–73.