Ht31, 1 mg

Background: This peptide is derived from amino acids 493-515 of the thyroid protein Ht31 and is predicted to form an amphipathic alpha helix. This helical region of the Ht31 protein binds to the dimerisation domain of type II regulatory subunits of the cAMP-dependent protein kinase (PKA) thus localizing this enzyme to specific subcellular sites. A number of so-called A-kinase anchoring proteins (AKAP) for a specific targeting to subcellular compartments are known and all bind to the PKA regulatory subunits with a similar alpha helical motif. The Ht31 peptide competes with binding between PKA R-subunits and AKAPs and demonstrates that specific interaction occurs.

Amino acid sequence: DLIEEAASRIVDAVIEQVKAAGA-OH
Molecular Weight: 2367.7 g/mol
Purity: 90% (HPLC, MS)
Appearance: Lyophilized white solid (TFA salt)

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.

Product specific literature references:

Scott JD, Stofko RE, McDonald JR, Comer JD, Vitalis EA, Mangili JA. (1990) "Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase." J. Biol. Chem. 265(35):21561-6

Carr DW, Stofko-Hahn RE, Fraser ID, Bishop SM, Acott TS, Brennan RG, Scott JD. (1991) "Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif." J. Biol. Chem. 266 (22): 14188-92.

Carr DW, Hausken ZE, Fraser ID, Stofko-Hahn RE, Scott JD. (1992) "Association of the type II cAMP-dependent protein kinase with a human thyroid RII-anchoring protein. Cloning and characterization of the RII-binding domain." J. Biol. Chem. 267(19):13376-82.

Product Citations:

Newell AE1, Fiedler SE, Ruan JM, Pan J, Wang PJ, Deininger J, Corless CL, Carr DW. (2008) "Protein kinase A RII-like (R2D2) proteins exhibit differential localization and AKAP interaction."Cell Motil Cytoskeleton.65(7):539-52.

Hermann JS, Skroblin P, Bertinetti D, Hanold LE, von der Heide EK, Wagener EM, Zenn HM, Klussmann E, Kennedy EJ, Herberg FW. (2015) "Neurochondrin is an atypical RIIα-specific A-kinase anchoring protein."Biochim Biophys Acta. 1854(10 Pt B):1667-75.

Tuettenberg A, Hahn SA, Mazur J, Gerhold-Ay A, Scholma J, Marg I, Ulges A, Satoh K, Bopp T, Joore J, Jonuleit H. (2016) "Kinome Profiling of Regulatory T Cells: A Closer Look into a Complex Intracellular Network." PLoS One. 16;11(2)