Ht31P, 1 mg

Background: 23 amino acid peptide derived from amino acids 493-515 of the thyroid protein Ht31. This negative control peptide contains two isoleucine to proline substitutions (I502P, I507P) which alters the helical structure of the peptide and abolishes binding to the regulatory subunits of PKA. The Ht31P peptide does not compete with binding between PKA R-subunits and AKAPs and thus works as a negative control for Ht31.

Amino acid sequence:     DLIEEAASRPVDAVPEQVKAAGA-OH
Molecular Weight:  2335.6 g/mol
Purity:  90% (HPLC, MS)
Appearance:  Lyophilized white solid (TFA salt)

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.

Product specific literature references:

JD, Stofko RE, McDonald JR, Comer JD, Vitalis EA, Mangili JA. (1990) "Type II regulatory subunit dimerization determines the subcellular localization of the cAMP-dependent protein kinase." J. Biol. Chem. 265(35):21561-6

Carr DW, Stofko-Hahn RE, Fraser ID, Bishop SM, Acott TS, Brennan RG, Scott JD. (1991) "Interaction of the regulatory subunit (RII) of cAMP-dependent protein kinase with RII-anchoring proteins occurs through an amphipathic helix binding motif." J. Biol. Chem. 266 (22): 14188-92.

Carr DW, Hausken ZE, Fraser ID, Stofko-Hahn RE, Scott JD. (1992) "Association of the type II cAMP-dependent protein kinase with a human thyroid RII-anchoring protein. Cloning and characterization of the RII-binding domain." J. Biol. Chem. 267(19):13376-82.

Product Citations:

Newell AE1, Fiedler SE, Ruan JM, Pan J, Wang PJ, Deininger J, Corless CL, Carr DW. (2008) "Protein kinase A RII-like (R2D2) proteins exhibit differential localization and AKAP interaction."Cell Motil Cytoskeleton.65(7):539-52.

Hermann JS, Skroblin P, Bertinetti D, Hanold LE, von der Heide EK, Wagener EM, Zenn HM, Klussmann E, Kennedy EJ, Herberg FW. (2015) "Neurochondrin is an atypical RIIα-specific A-kinase anchoring protein."Biochim Biophys Acta. 1854(10 Pt B):1667-75.

Tuettenberg A, Hahn SA, Mazur J, Gerhold-Ay A, Scholma J, Marg I, Ulges A, Satoh K, Bopp T, Joore J, Jonuleit H. (2016) "Kinome Profiling of Regulatory T Cells: A Closer Look into a Complex Intracellular Network." PLoS One. 16;11(2)