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Recombinant Human TRAILR1 /DR4 /TNFRSF10A Protein, C-Fc Tag, 100µg  

Recombinant Human TRAILR1 /DR4 /TNFRSF10A Protein, C-Fc Tag, 100µg

Recombinant Human TRAILR1 /DR4 /TNFRSF10A Protein, Ala 24 - Asn 239, expressed from human 293 cells (HEK293), Fc Tag

Synonym
recombinant , human protein, TNFRSF10A, TRAIL-R1, CD261, APO2, DR4

More details

TR1-H5254-100

Availability: within 7 days

312,00 €

Background
Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily. TRAILR1 / TNFRSF10A contains 1 death domain and 3 TNFR-Cys repeats. TNFRSF10A / DR4 is widely expressed and high levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells. APO2 / TNFRSF10A is receptor for the cytotoxic ligand TNFSF10 / TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. TRAILR-1 / DR4 / CD261 promotes the activation of NF-kappa-B.

Source
Recombinant Human TRAIL R1, Fc Tag (TR1-H5254) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Asn 239 (Accession # NP_003835).
Predicted N-terminus: Ala 24

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 49.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhTRAILR1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human TRAIL R1, Fc Tag (Cat. No. TR1-H5254) at 5 μg/mL (100 μL/well) can bind Human TRAIL, Mouse IgG2a Fc Tag (Cat. No. TRL-H5259) with a linear range of 4-16 ng/mL (QC tested).

Loaded Human TRAIL R1, Fc Tag (Cat. No. TR1-H5254) on AHC Biosensor, can bind Human TRAIL, His Tag with an affinity constant of 0.891 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Loaded Human TRAIL, His Tag on HIS1K Biosensor, can bind Human TRAIL R1, Fc Tag (Cat. No. TR1-H5254) with an affinity constant of 2.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

References

(1) Chaudhary P.M., et al., 1997, Immunity 7:821-830.
(2) Simova S., et al., 2008, Apoptosis 13:423-436.