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Recombinant SARS-CoV-2 S1 protein (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G), Fc Tag, 100µg  

Recombinant SARS-CoV-2 S1 protein (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G), Fc Tag, 100µg

Recombinant SARS-CoV-2 S1 protein (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G), known as B.1.351 or 20C/501Y.V2, AA Val 16 - Arg 685, expressed from human 293 cells (HEK293) Fc Tag

Synonyms: Recombinant, Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1

More details

S1D-C5256-100

Availability: within 10-14 days

494,00 €

Background
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S1 protein, Fc Tag (S1D-C5256) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). The mutations (L18F, D80A, D215G, LAL242-244del, R246I, K417N, E484K, N501Y, D614G) were identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2).
Predicted N-terminus: Val 16

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 101.1 kDa. The protein migrates as 125-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Molecular recognition of SARS-CoV-2 spike protein with three essential partners: exploring possible immune escape mechanisms of viral mutants"
Cheng, Zheng, Zhang et al
J Mol Model (2023) 29 (4), 109
(2) "Cytotoxic T Cells Targeting Spike Glycoprotein Are Associated with Hybrid Immunity to SARS-CoV-2"
Phan, Layton, Yu et al
J Immunol (2023)
(3) "Using low-cost disposable immunosensor based on flexible PET screen-printed electrode modified with carbon black and gold nanoparticles for sensitive detection of SARS-CoV-2"
Ferreira, Reis, Martins et al
Talanta Open (2023) 7, 100201
Showing 1-3 of 2603 papers.