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Recombinant human ANGPTL7 / CDT6 Protein, His Tag, 100 µg  

Recombinant human ANGPTL7 / CDT6 Protein, His Tag, 100 µg

Recombinant human ANGPTL7, Gln 27 - Pro 346, expressed from human 293 cells (HEK293), His Tag

Synonym
recombinant, human, ANGPTL7,CDT6 protein

More details

AN7-H52H5-100

492,00 €

Background
Angiopoietin-like 7 (ANGPTL7) is also known as Corneal-Derived Transcript 6 (CDT6), and is a secreted glycoprotein that is structurally related to the angiopoietins. It is expressed in the corneal stroma, trabecular meshwork, and sclera and is elevated in glaucoma aqueous humor. Recently, ANGPTL proteins, including ANGPTL-2, -3, -4, -5 and -7, are identified as growth factors in combination with SCF, Thrombopoietin, IGF-II and FGF acidic that enhance the expansion and engraftment of human and mouse hematopoietic stem cells.

Source
Recombinant human ANGPTL7 protein, His Tag (AN7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Pro 346 (Accession # O43827-1).
Predicted N-terminus: Gln 27

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39 kDa. The protein migrates as 38-40 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human ANGPTL7, His Tag (Catalog # AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Human LILRB4, Fc Tag with a linear range of 0.031-0.5 μg/mL.

Measured by its binding ability in a functional ELISA. Immobilized Human ANGPTL7, His Tag (Catalog # AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Human LILRB2, Fc Tag with a linear range of 0.031-2 μg/mL.

Measured by its binding ability in a functional ELISA. Immobilized Human ANGPTL7, His Tag (Catalog # AN7-H52H5) at 5 μg/mL (100 μL/well) can bind Human LILRA3, Fc Tag with a linear range of 0.016-1 μg/mL. 

References

(1) Peek R., et al., 1998, Invest. Opthalmol. Visual Sci. 39 (10): 1782-1788.
(2) Peek R., et al., 2002, J. Biol. Chem. 277 (1): 686-693.
(3) Kuchtey, J. et al., 2008, Invest. Opthalmol. Visual Sci. 49 (8): 3438-3448.