Recombinant Human Serpin A3 Protein, His Tag, 50 µg

Background
Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Source
Recombinant Human Serpin A3, His Tag (SE3-H5221) is expressed from human 293 cells (HEK293). It contains AA His 24 - Ala 423 (Accession # AAH03559).
Predicted N-terminus: His 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "Longitudinal Fluctuations in Protein Concentrations and Higher-Order Structures in the Plasma Proteome of Kidney Failure Patients Subjected to a Kidney Transplant"
Kalaidopoulou Nteak, Völlmy, Lukassen et al
J Proteome Res (2024) 23 (6), 2124-2136
(2)  "The role of the immune system in depersonalisation disorder"
Zheng, Feng, Song et al
World J Biol Psychiatry (2024) 25 (5), 291-303
(3)  "Discovery of Alpha-1-Antichymotrypsin as a Marker of Delayed Recovery from Concussion in Children"
Swaney, Babl, Rausa et al
J Neurotrauma (2024)
Showing 1-3 of 2115 papers.