Recombinant Human Serpin A3 Protein, His Tag, 50 µg

Background
Serpin A3 is also known as Alpha-1-antichymotrypsin (ACT or AACT), Cell growth-inhibiting gene 24/25 protein, which belongs to the serpin family. Serpin A3 can bind DNA. Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

Source
Recombinant Human Serpin A3, His Tag (SE3-H5221) is expressed from human 293 cells (HEK293). It contains AA His 24 - Ala 423 (Accession # AAH03559). Predicted N-terminus: His 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.6 kDa. The protein migrates as 50-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "Pre-clinical evaluation of antiproteases as potential candidates for HIV-1 pre-exposure prophylaxis"
Herrera, Olejniczak, Noël-Romas et al
Front Reprod Health (2022) 4, 998913
(2)  "The Search for Associations of Serum Proteins with the Presence of Unstable Atherosclerotic Plaque in Coronary Atherosclerosis"
Stakhneva, Kashtanova, Polonskaya et al
Int J Mol Sci (2022) 23 (21)
(3)  "Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin"
Kim, Leng, Park et al
Nat Commun (2022) 13 (1), 6581
Showing 1-3 of 2096 papers.