Recombinant Human Serpin H1 Protein, His Tag, 50µg

Background
Serpin H1 is also known as SERPINH1, 47 kDa heat shock protein (HSP47), Collagen-binding protein (Colligin), Arsenic-transactivated protein 3 (AsTP3), which belongs to the serpin family. SERPINH1 binds specifically to collagen. SERPINH1 / HSP47 could be involved as a chaperone in the biosynthetic pathway of collagen.

Source
Recombinant Human Serpin H1, His Tag (SE1-H5224) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Leu 418 (Accession # P50454-1).
Predicted N-terminus: Ala 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 45.5 kDa. The protein migrates as 48-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1)  "Intergenerational plasticity to cycling high temperature and hypoxia affects offspring stress responsiveness and tolerance in zebrafish"
Lim, Bernier
J Exp Biol (2023) 226 (16)
(2)  "Neuro-immunohistochemical and molecular gene expression variations during hibernation and activity phases between Rana mascareniensis and Rana ridibunda"
Rabeae, Mahfouz, Abdel Latif et al
J Therm Biol (2023) 114, 103490
(3) "Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma"
da Costa, Dourado, de Moraes et al
J Oral Pathol Med (2023) 52 (7), 601-609
Showing 1-3 of 673 papers.