Recombinant Human Arginase 1 / ARG1 Protein, His Tag, 50 µg

Background
Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1 / ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1 / ARG1 are the cause of argininemia (ARGIN).

Source
Recombinant Human Arginase 1, His Tag (AR1-H5228) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Lys 322 (Accession # NP_000036).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 35.8 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Three neo-Clerodane Diterpenoids from Tinospora cordifolia Stems and Their Arginase Inhibitory Activities"
Hoang, Hoshino, Kodama et al
Chem Pharm Bull (Tokyo) (2024) 72 (6), 540-546
(2) "Neoadjuvant toripalimab combined with axitinib in patients with locally advanced clear cell renal cell carcinoma: a single-arm, phase II trial"
Huang, Wang, Xu et al
J Immunother Cancer (2024) 12 (6)
(3) "Antitumor activity of dictamnine against colorectal cancer through induction of ferroptosis and inhibition of M2 macrophage polarization via the MAPK signaling"
Zuo, Lin, Song et al
Arch Biochem Biophys (2024)
Showing 1-3 of 5253 papers.