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Recombinant Fc gamma RIII / CD16 Protein, His Tag (SPR verified), 100µg  

Recombinant Fc gamma RIII / CD16 Protein, His Tag (SPR verified), 100µg

Recombinant Mouse Fc gamma RIII / CD16 Protein, Leu 32 - Thr 215, expressed from human 293 cells (HEK293), His Tag (SPR verified)

Synonyms: recombinant mouse protein FCGR3, CD16

More details

CDA-M52H8-100

Availability: within 7 days

336,00 €

Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Source
Recombinant Mouse CD16, His Tag (SPR verified) (CDA-M52H8) is expressed from human 293 cells (HEK293). It contains AA Leu 32 - Thr 215 (Accession # Q5D5I8).
Predicted N-terminus: Leu 32

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity-SPR
Mouse CD16, His Tag (SPR verified) (Cat. No. CDA-M52H8) Captured on NTA-Series S sensor chip can bind OKT3 with an affinity constant of 0.126 μM as determined in a SPR assay (Biacore T200) (QC tested).

Bioactivity-BLI
Loaded Mouse CD16, His Tag (SPR verified) (Cat. No. CDA-M52H8) on NTA Biosensor, can bind OKT3 with an affinity constant of 0.78 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Loaded Mouse CD16, His Tag (SPR verified) (Cat. No. CDA-M52H8) on NTA Biosensor, can bind Herceptin with an affinity constant of 2.8 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

References

(1) Webster NL., et al., 2006, J Leukoc Biol. 79: 294-302.
(2) Nagarajan S., et al., 1995, J Biol Chem. 270: 25762-70.
(3) Ravetch JV., 1989, J. Exp. Med. 170:481.

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