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Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source
Recombinant SARS-CoV-2 S1 protein CTD, His Tag (MALS-verified) (S1D-C52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 334 - Pro 527 (Accession # QHD43416.1).
Predicted N-terminus: Asn 334
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 23.6 kDa. The protein migrates as 28-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.3. Normally Trehalose are added as protectants before lyophilization.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Bioactivity
Please refer to product data sheet.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
(1) "Characterization of SARS-CoV-2 omicron variants from Iran and evaluation of the effect of mutations on the spike, nucleocapsid, ORF8, and ORF9b proteins function"
Ahmadi, Shahbazi, Zakeri et al
J Biomol Struct Dyn (2022)
(2) "Field Evaluation of Wheat Varieties Using Canopy Temperature Depression in Three Different Climatic Growing Seasons"
Chai, Zhao, Lu et al
Plants (Basel) (2022) 11 (24)
(3) "A Monoclonal Antibody Targeting C-Terminal Domain of Transmissible Gastroenteritis Virus Spike Protein"
Liu, Li
Monoclon Antib Immunodiagn Immunother (2022) 41 (6), 328-332
Showing 1-3 of 46 papers.
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