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The synthetic peptide RRRDDDSDDD can be used as a substrate for protein kinase CK2 alpha subunit or holoenzyme in in vitro kinase assays. It is phosphorylated by CK2 holoenzyme with a Km of 50 µM.
CK2 substrate peptide: order-no. PEP-CK2I-025
Amino acid sequence: RRRDDDSDDD (10-mer)
Molecular Weight: 1264 g/mol
Shipment conditions: Ambient temperature
Long Term Storage: -20°C (AVOID FREEZE/THAW CYCLES)
Purity: 90-95% (by HPLC)
Appearance: Lyophilized solid
Handling: Reconstitute in water dest.
Meggio F, Marin O, Pinna LA. (1994) Substrate specificity of protein kinase CK2. Cell Mol Biol Res 40, 401-9
Bosc DG, Luscher B, Litchfield DW (1999) "Expression and regulation of protein kinase CK2 during the cell cycle" Mol Cell Biochem. 191(1-2):213-22
McKendrick L, Milne D, Meek D (1999) "Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable" Mol Cell Biochem. 191(1-2):187-99
Winiewska M., Makowska M., Maj P., Wielechowska M., Bretner M., Poznański J., Shugar D.(2015) "Thermodynamic parameters for binding of some halogenated inhibitors of human protein kinase CK2." Biochem Biophys Res Commun. 456(1):282-7.
Hochscherf J, Lindenblatt D, Steinkrüger M, Yoo E, Ulucan Ö, Herzig S, Issinger OG, Helms V, Götz C, Neundorf I, Niefind K, Pietsch M. (2015) "Development of a high-throughput screening-compatible assay to identify inhibitors of the CK2α/CK2β interaction." Anal Biochem. 468:4-14.
PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.
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