CK2 peptide substrate, 25 mg

The synthetic peptide RRRDDDSDDD can be used as a substrate for protein kinase CK2 alpha subunit or holoenzyme in in vitro kinase assays. It is phosphorylated by CK2 holoenzyme with a Km of 50 µM.

CK2 substrate peptide:    order-no. PEP-CK2I-025
Amino acid sequence:      RRRDDDSDDD (10-mer)
Molecular Weight:            1264 g/mol
Shipment conditions:        Ambient temperature
Long Term Storage:        -20°C (AVOID FREEZE/THAW CYCLES)
Purity:                               90-95% (by HPLC)
Appearance:                     Lyophilized solid
Handling:                          Reconstitute in water dest.

Product specific literature references:

Meggio F, Marin O, Pinna LA. (1994) Substrate specificity of protein kinase CK2. Cell Mol Biol Res 40, 401-9

Bosc DG, Luscher B, Litchfield DW (1999) "Expression and regulation of protein kinase CK2 during the cell cycle" Mol Cell Biochem. 191(1-2):213-22

McKendrick L, Milne D, Meek D (1999) "Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable" Mol Cell Biochem. 191(1-2):187-99

Product Citations:

Winiewska M^., Makowska M^., Maj P., Wielechowska M^., Bretner M., Poznański J., Shugar D.(2015) "Thermodynamic parameters for binding of some halogenated inhibitors of human protein kinase CK2." Biochem Biophys Res Commun. 456(1):282-7.

Hochscherf J, Lindenblatt D, Steinkrüger M, Yoo E, Ulucan Ö, Herzig S, Issinger OG, Helms V, Götz C, Neundorf I, Niefind K, Pietsch M. (2015) "Development of a high-throughput screening-compatible assay to identify inhibitors of the CK2α/CK2β interaction." Anal Biochem. 468:4-14.

PLEASE NOTE: This product is designed for research purposes and can only be delivered to academic and business customers.