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Recombinant MERS S1 protein, His Tag, 100µg  

Recombinant MERS S1 protein, His Tag, 100µg

Recombinant MERS S1 protein, His Tag, AA Tyr 18 - Arg 751, expressed from human 293 cells (HEK293), His Tag

Synonyms: Recombinant, Spike, S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, COVID-19

More details

S1N-M52H5-100

Availability: within 7 days

494,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant MERS S1 protein, His Tag (S1N-M52H5) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Arg 751 (Accession # K0BRG7-1).
Predicted N-terminus: Tyr 18

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 83.1 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Modeling foot sole cutaneous afferents: FootSim"
Katic, Siqueira, Cleland et al
iScience (2023) 26 (1), 105874
(2) "Identification of Kaempferol as viral entry inhibitor and DL-Arginine as viral replication inhibitor from selected plants of Indian traditional medicine against COVID-19: An in silico guided in vitro approach"
J, Murali, Nair et al
Curr Comput Aided Drug Des (2023)
(3) "An untargeted metabolomic approach to investigate antiviral defence mechanisms in memory leukocytes secreting anti-SARS-CoV-2 IgG in vitro"
Federica, Giuseppina, Veronica et al
Sci Rep (2023) 13 (1), 629
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