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Recombinant human Fc gamma RIIB /CD32b Protein, His tag, 100µg  

Recombinant human Fc gamma RIIB /CD32b Protein, His tag, 100µg

Recombinant Human Fc gamma RIIB /CD32b Protein (rhCD32b), produced in human HEK293 cells, His-Tag

Synonym
recombinant, human, protein, FCGR2B, CD32b, CD32, FCG2

More details

CDB-H5228-100

Availability: within 7 days

336,00 €

Background
Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. Low affinity immunoglobulin gamma Fc region receptor II-b (FCGR2B) is also known as CD32b, FCG2, IGFR2. CD32B is expressed on B cells and myeloid dendritic cells. Ligation of CD32B on B cells downregulates antibody production and may, in some circumstances, promote apoptosis. Co-ligation of CD32B on dendritic cells inhibits maturation and blocks cell activation. CD32B may also be a target for monoclonal antibody therapy for malignancies.

Source
Human Fc gamma RIIIB / CD16b (NA2), His Tag (CDB-H5222) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Ser 200 (Accession # O75015-1). The NA2 form of the CD16B (CDB-H5222) differ with the NA1 form of CD16B in AA20, 49, 66, and 90. The NA2 form carries S20, S49, N66, and I90, while the NA1 form (CDB-H82E4 / CDB-H5227) carries R20, N49, D66, and V90.
Predicted N-terminus: Gly 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 22.7 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCD32b by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Human FOLR1, Fc Tag (Cat. No. FO1-H5253) with a linear range of 10-78 ng/mL (QC tested).

References

(1) Hamada F., et al., 1993, Proc. Natl. Acad. Sci. U.S.A. 90:6305-6309.
(2) Sarmay G., et al., 1997, Immunol. Lett. 57:159-164.
(3) Laine D., et al., 2005, J. Gen. Virol. 86:1771-1784.