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Recombinant SARS-CoV-2 S protein (B.1.617), trimer, His Tag (MALS verified), 50µg  

Recombinant SARS-CoV-2 S protein (B.1.617), trimer, His Tag (MALS verified), 50µg

Recombinant SARS-CoV-2 Spike (T95I, G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H), AA Val 16 - Pro 1213, expressed from human 293 cells (HEK293), trimer, His Tag (MALS verified)

Synonyms: Recombinant, protein, S protein, Spike glycoprotein, S glycoprotein, COVID-19

More details

SPN-C52Hr-50

Availability: within 7 days

540,00 €

Background
It's been reported that Coronavirus  can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 Spike Trimer, His Tag (SPN-C52Hr) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The mutations (T95I, G142D, E154K, L452R, E484Q, D614G, P681R) were identified in the SARS-CoV-2 variants which emerged in India (known as B.1.617). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Predicted N-terminus: Val 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.2 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution

References

  • "Rapid Increase of SARS-CoV-2 Variant B.1.1.7 Detected in Sewage Samples from England between October 2020 and January 2021" Wilton, Bujaki, Klapsa et al. mSystems (2021)

  • "COVID-19 Spike Protein Induced Phononic Modification in Antibody-Coupled Graphene for Viral Detection Application" Nguyen, Kim, Lindemann et al. ACS Nano (2021)

  • "Preliminary evidence of blunted humoral response to SARS-CoV-2 mRNA vaccine in multiple sclerosis patients treated with ocrelizumab" Gallo, Capuano, Donnarumma et al. Neurol Sci (2021)