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Recombinant human BACE1 Protein, Fc Tag, 200µg  

Recombinant human BACE1 Protein, Fc Tag, 200µg

Recombinant human BACE1 Protein, With N-Fc Tag (rhBACE1-Fc) Thr22-Thr457 was produced in human 293 cells (HEK293)

Synonym: recombinant, human, protein BACE1, ASP2, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1

More details

BA1-H5261-200

Availability: within 7 days

276,00 €

Background
Beta-secretase 1 (BACE1) also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer\'s disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer\'s patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. [2-3]

Source
Recombinant human BACE1 Protein, With N-Fc Tag (rhBACE1-Fc) Thr22-Thr457 (Accession # NP_036236.1 ) was produced in human 293 cells (HEK293)

Molecular Characterization
rhBACE1-Fc, fused with the Fc region of human IgG1 at the N-terminus and has a calculated MW of 75 kDa. The predicted N-terminus of BACE1 is Thr22. DTT-reduced protein migrates as 100-110 kDa polypeptide in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhBACE1-Fc by the LAL method.

Purity
>85% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK (Dpn) RR-NH2, The specific activity is > 1.5 pmoles /min /μg.

References

(1) Willem M, et al., 2006, Science 314 (5799): 664–6.
(2) Zacchetti D, et al., 2007, Neurodegener Dis. 4: 117-126.
(3) Zhao J. et al., 2007, J. Neurosci. 27: 3639- 3649.