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Recombinant human BACE1 Protein, Fc Tag, 200µg  

Recombinant human BACE1 Protein, Fc Tag, 200µg

Recombinant human BACE1 Protein, With N-Fc Tag (rhBACE1-Fc) Thr22-Thr457 was produced in human 293 cells (HEK293)

Synonym: recombinant, human, protein BACE1, ASP2, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2

More details

BA1-H5261-200

Availability: within 7 days

276,00 €

Background
Beta-secretase 1 (BACE1) also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer\'s disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer\'s patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. [2-3]

Source
Recombinant human BACE1 Protein, Fc Tag (BA1-H5261) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1).
Predicted N-terminus: Thr 22 & Glu 46

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The Human BACE-1 will be further processed into mature form (Glu 46-Thr 457). The protein has a calculated MW of 75.2 kDa (pro-form) and 72.6 kDa (mature-form). The protein migrates as 80-96 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1)"Ameliorative Effects by Hexagonal Boron Nitride Nanoparticles against Beta Amyloid Induced Neurotoxicity"
Aydin, Turkez, Tozlu et al
Nanomaterials (Basel) (2022) 12 (15)
(2)"A Coumarin-donepezil Hybrid as a Blood-brain Barrier Permeable Dual Cholinesterase Inhibitor: Isolation, Synthetic Modifications and Biological Evaluation of Natural Coumarins"
Sharma, Nuthakki, Gairola et al
ChemMedChem (2022)
(3) "Friedelin Attenuates Neuronal Dysfunction and Memory Impairment by Inhibition of the Activated JNK/NF-κB Signalling Pathway in Scopolamine-Induced Mice Model of Neurodegeneration"
Sandhu, Irfan, Shah et al
Molecules (2022) 27 (14)
Showing 1-3 of 629 papers.