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Recombinant human BACE1 Protein, tag free, 100µg  

Recombinant human BACE1 Protein, tag free, 100µg

Recombinant Human BACE1 Protein, Native (rhBACE1) Thr22-Thr 457,  produced in human 293 cells (HEK293), tag free

Synonym: recombinant, human, protein BACE1, ASP2, BACE, Beta-secretase-1, memapsin-2, aspartyl-protease-2, beta-site-APP-cleaving-enzyme-1

More details

BA1-H5213-100

Availability: within 7 days

336,00 €

Background
Beta-secretase 1 (BACE1), also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer\'s disease, and in the formation of myelin sheaths in peripheral nerve cells.[1] The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer\'s patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD. [2-3]

Source
Recombinant Human BACE1 Protein, Native (rhBACE1) Thr22-Thr 457(Accession # NP_036236.1 ) was produced in human 293 cells (HEK293).

Molecular Characterization
rhBACE1 contains no “tags” and has a calculated MW of 49 kDa. The predicted N-terminus is Thr22. DTT-reduced protein migrates as 50-65 kDa polypeptide in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhBACE1 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
N/A

References

(1) Willem M, et al., 2006, Science 314 (5799): 664–6.
(2) Zacchetti D, et al., 2007, Neurodegener Dis. 4: 117-126.
(3) Zhao J. et al., 2007, J. Neurosci. 27: 3639- 3649.