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Recombinant human IL13RA1 /CD213A1 Protein, His Tag, 100µg  

Recombinant human IL13RA1 /CD213A1 Protein, His Tag, 100µg

Human IL-13 R alpha 1 Protein, His Tag (SPR verified)

Synonym
Recombinant Human Protein, IL13RA1, CD213A1, IL-13Ra, NR4, RP13-128O4.2, IL13Rα1

More details

IL1-H5224-100

Availability: within 7 days

378,00 €

Background
Interleukin 13 receptor, alpha 1, also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.[1-3]

Source
Human IL-13 R alpha 1, His Tag (SPR verified) (IL1-H5224) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Thr 343 (Accession # NP_001551.1).
Predicted N-terminus: Gly 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 38.0 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL13RA1 by the LAL method.

Purity
>98% purity as determined by SDS-PAGE of reduced rhIL13RA1.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C). 

References

(1) Caput, D. et al., 1996, J. Biol. Chem. 271:16921.
(2) Donaldson, D.D. et al., 1998, J. Immunol. 161:2317.
(3) Hilton, D.J. et al., 1996, Proc. Natl. Acad. Sci. USA 93:497.