Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human IL4R /CD124 Protein, His Tag, 50µg  

Recombinant human IL4R /CD124 Protein, His Tag, 50µg

Recombinant Human IL-4 R alpha / CD124 Protein, Met 26 - His 232 was produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Human Protein, IL4R, CD124, IL4RA

More details

ILR-H5221-050

Availability: within 7 days

336,00 €

Background
IL-4 is a pleiotropic cytokine produced by activated Th2 cells and mast cells, and plays a pivotal role in immune responses. The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells. Hematopoietic cellular responses to IL-4 are mediated by a high affinity receptor complex comprised of the 140 kDa IL4Rα (CD124)subunit and the 70 kDa common cytokine γc chain (CD132). Interleukin 4 Receptor (IL4R) also known as CD124, IL4Rα and BSF receptor, is a type I cytokine receptor produced by activated Th2 cells and mast cells, and plays an important role in Th2-biased immune responses, alternative macrophage activation, mucosal immunity, allergic inflammation, tumor progression, and atherogenesis. A soluble form of the encoded IL4R protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. IL4R can alternatively associate with IL13Ra1 to form the type II receptor which is responsive to both IL4 and IL13. Interleukin-4 receptor has been shown to interact with SHC1.[1-5]

Source
Recombinant Human IL4R /CD124 Protein, His Tag (ILR-H5221) is expressed from human 293 cells (HEK293). It contains AA Met 26 - His 232 (Accession # NP_000409.1).
Predicted N-terminus: Met 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 24.6 kDa. The protein migrates as 36-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhIL4R by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Cadmium exposure triggers oxidative stress, necroptosis, Th1/Th2 imbalance and promotes inflammation through the TNF-α/NF-κB pathway in swine small intestine
Chen, Bi, Yang et al
J Hazard Mater (2021) 421, 126704
(2) Alpha-Lipoic Acid Protects Co-Exposure to Lead and Zinc Oxide Nanoparticles Induced Neuro, Immuno and Male Reproductive Toxicity in Rats
Deore, S, Naqvi et al
Front Pharmacol (2021) 12, 626238
(3) Dysregulation of the Adaptive Immune System in Patients With Early-Stage Parkinson Disease
Yan, Yang, Wei et al
Neurol Neuroimmunol Neuroinflamm (2021) 8 (5)

Showing 1-3 of 927 papers.
Please refer to product data sheet.