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Recombinant human GCSFR /CD114 Protein, His Tag, 100µg  

Recombinant human GCSFR /CD114 Protein, His Tag, 100µg

Recombinant Human GCSFR /CD114 Protein (rhGCSFR) Glu25-Pro621 produced in human 293 cells (HEK293), His Tag

Synonym
recombinant human protein, RP11-328D5.2, CD34

More details

GCR-H5223-100

Availability: within 7 days

300,00 €

 Background
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I? membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis.[1] Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.[2] Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.[3]

Source
Recombinant Human GCSFR /CD114 Protein (rhGCSFR) Glu25-Pro621 (Accession # NP_000751.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhGCSFR, fused with 6×his tag at the N-terminus, has a calculated MW of 69 kDa expressed. The predicted N-terminus is Glu25. Protein migrates as 94 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhGCSFR by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4C-8C); After reconstitution under sterile conditions for 1 month (4C-8C) or 3 months (-20C to -70C).

Bioactivity
n/a

References

(1) Fan Dong, Andrew C. Larner, 2000, BLOOD, VOLUME 95(5).
(2) Zeidler C, Welte K, 2002, Semin. Hematol. 39 (2): 82–8.
(3) Beekman R, Touw IP, 2010, Beekman R, Touw IP.