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Recombinant human GCSFR /CD114 Protein, Fc Tag, 200µg  

Recombinant human GCSFR /CD114 Protein, Fc Tag, 200µg

Recombinant Human GCSFR /CD114 Protein, Glu25-Pro621, produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant Human Protein, CSF3R, CD114, GCSFR, GCSF-R, G-CSF-R, granulocyte-colony-stimulating-factor-receptor

More details

GCR-H5250-200

Availability: within 7 days

348,00 €

Background
Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as Cluster of Differentiation 114 (CD114), CSF3R and GCSF, is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF), a cytokine that plays a critical role in the regulation of the activation, proliferation, differentiation, and survival of the neutrophilic granulocyte lineage. G-CSFR belongs to a family of cytokine receptors known as the hematopoietin receptor family. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type I II (FNIII) domains in the extracellular region. G-CSFR is present mainly on precursor cells in the bone marrow, and, in response to stimulation by G-CSF, initiates cell proliferation and differentiation into mature neutrophilic granulocytes and macrophages. G-CSFR mediates the specific effect of GCSF through activating a variety of intracellular signaling cascades, including the Jak/Stat, PI3/Akt, Ras-Raf-MAP kinase, and Src family kinase pathways, and thus functions in defense against infection, inflammation and repair, and in the maintenance of steady state hematopoiesis.[1] Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.[2] Mutations in the intracellular part of this receptor are also associated with certain types of leukemia.[3]

Source
Recombinant Human GCSFR /CD114 Protein, With C-Fc Tag (rhG-CSFR-Fc) Glu25-Pro621 (Accession # NP_000751.1) was produced in human 293 cells (HEK293)

Molecular Characterization
rhG-CSFR-Fc, fused with Fc region of human IgG1 at C-terminus and has a calculated MW of 92.8 kDa expressed. The predicted N-terminus is Glu 25. Protein migrates as 100-130 kDa in reduced SDS-PAGE resulting from glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhG-CSFR-Fc by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH7.0. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human G-CSF, Tag Free (Cat. No. GCF-H5214) at 5 μg/mL (100 μL/well) can bind Human G-CSF R, Fc Tag (Cat. No. GCR-H5250) with a linear range of 0.5-5 ng/mL (QC tested).

References

(1) Fan Dong, Andrew C. Larner, 2000, BLOOD, VOLUME 95(5).
(2) Zeidler C, Welte K, 2002, Semin. Hematol. 39 (2): 82–8.
(3) Beekman R, Touw IP, 2010, Beekman R, Touw IP.