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Recombinant human CEACAM1 /CD66a /BGP1 Protein, 100µg  

Recombinant human CEACAM1 /CD66a /BGP1 Protein, 100µg

Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a) Gln 35 - Gly 428 was produced in human 293 cells (HEK293), His Tag

Synonym: recombinant, human, protein CEACAM1, CD66a, BGP, BGP1, BGPI

More details

CE1-H5220-100

Availability: within 7 days

300,00 €

Background
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1 /CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1 /CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Source
Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a), His Tag (CE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gly 428 (Accession # AAH14473).
Predicted N-terminus: Gln 35

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 57-100 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCEACAM1 /CD66a by the LAL method.

Purity
>92% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) "CEACAM-1 Induced CSF3-receptor Downregulation in Bone Marrow Associated With Refractory Neutropenia in Advanced Cirrhosis"
Bihari, Baweja, Shasthry et al
J Clin Transl Hepatol (2022) 10 (1), 53-62
(2) "Immunoglobulin A nephropathy is characterized by anticommensal humoral immune responses"
Currie, Coburn, Porfilio et al
JCI Insight (2022) 7 (5)
(3) "Exopolysaccharides from Lactobacillus delbrueckii ssp. bulgaricus OLL1073R-1 prevent influenza virus infection and attenuate secondary bacterial infection risk"
Ishikawa, Kuno, Kohda et al
Lett Appl Microbiol (2022)
Showing 1-3 of 58 papers.