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Recombinant human CEACAM1 /CD66a /BGP1 Protein, 100µg  

Recombinant human CEACAM1 /CD66a /BGP1 Protein, 100µg

Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a) Gln 35 - Gly 428 was produced in human 293 cells (HEK293)

Synonym: recombinant, human, protein CEACAM1, CD66a, BGP, BGP1, BGPI

More details

CE1-H5220-100

Availability: within 7 days

300,00 €

Background
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1 /CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1 /CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Source
Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a) Gln 35 - Gly 428 (Accession # AAH14473) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCEACAM1 /CD66a, fused with 6×His tag at the C-terminus, has a calculated MW of 44.2 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 60-130 kDa due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCEACAM1 /CD66a by the LAL method.

Purity
>92% purity as determined by SDS-PAGE.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

References

(1) Kuroki M., et al., 1991, Biochem. Biophys. Res. Commun. 176:578-585.
(2) Watt S.M., et al., 1994, Blood 84:200-210.
(3) Chen R., et al., 2009, J. Proteome Res. 8:651-661.
(4) Hoek KS., et al., 2008, Pigment Cell Melanoma Res. 21 (6): 665–76.