Recombinant human CEACAM1 /CD66a /BGP1 Protein, 100µg

Background
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), also known as Biliary glycoprotein 1 (BGP1), CD66a, which belongs to the immunoglobulin superfamily or CEA family. CEACAM1 /CD66a contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CEACAM1 /CD66a was described as an adhesion molecule mediating cell adhesion via both homophilic and heterophilic manners, and was detected on leukocytes, epithelia, and endothelia. Studies have revealed that CEACAM1 / BGP-1 performs actions in multiple cellular processes including tissue differentiation, angiogenesis, apoptosis, metastasis, as well as the modulation of innate and adaptive immune responses.

Source
Recombinant Human CEACAM1 /CD66a /BGP1 Protein (rhCEACAM1 /CD66a), His Tag (CE1-H5220) is expressed from human 293 cells (HEK293). It contains AA Gln 35 - Gly 428 (Accession # AAH14473).
Predicted N-terminus: Gln 35

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 57-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>92% purity as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The role of CEACAMs versus integrins in Helicobacter pylori CagA translocation: a systematic review"
Saberi, Shans, Ghaffari et al
Microbes Infect (2024) 26 (1-2), 105246
(2) "Immunological Responses Associated With Neoadjuvant Therapy in the Tumor Microenvironment of Esophageal Squamous Cell Carcinoma"
Takehara, Tamaki, Mimura et al
Anticancer Res (2023) 43 (10), 4691-4700
(3) "Correlation between human colon cancer specific antigens and Raman spectra. Attempting to use Raman spectroscopy in the determination of tumor markers for colon cancer"
Depciuch, Jakubczyk, Paja et al
Nanomedicine (2023) 48, 102657
Showing 1-3 of 65 papers.