Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human Fas Ligand/FasL Protein, 50µg  

Recombinant Human Fas Ligand/FasL Protein, 50µg

Recombinant Human Fas Ligand /FasL Protein (rhFas Ligand ) Pro 134 - Leu 281 was produced in human 293 cells (HEK293).

Synonym
recombinant human protein, FASLG, ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, TNFSF6, Fas ligand

More details

FAL-H5241-050

Availability: within 7 days

492,00 €

Background
Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Mature human Fas Ligand consists of a 179 amino acid (aa) extracellular domain (ECD), a 22 aa transmembrane segment, and a 80 aa cytoplasmic domain. Within the ECD, human Fas Ligand shares 81% and 78% aa sequence identity with mouse and rat Fas Ligand, respectively. Apoptosis triggered by Fas-Fas ligand binding plays a fundamental role in the regulation of the immune system. Its functions include:T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lymphoproliferative syndrome (ALPS), a childhood disorder of apoptosis.[1-3]

Source
Recombinant Human Fas Ligand /FasL Protein (rhFas Ligand ) Pro 134 - Leu 281(Accession # AAH17502.1) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhFasL by the LAL method.

Purity
>95% purity as determined by SDS-PAGE of reduced rhFasL.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human Fas Ligand, His Tag (Catalog # FAL-H5241) at 5μg/mL (100 μL/well) can bind Human Fas, Fc Tag (Catalog # FAS-H5252) with a linear range of 1.56-12.5 ng/mL.

References

(1) Sheikh MS, Fornace AJ., 2000, Leukemia 14 (8): 1509–1513.
(2) Andersen MH, et al., 2006, J. Invest. Dermatol. 126 (1): 32–41.
(3) Igney FH, Krammer PH., 2005, Cancer Immunol. Immunother. 54 (11): 1127–1136.