Recombinant Mouse EGFR /ErbB1 Protein, His Tag, 50µg

Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.[1] The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.[2]

Source
Recombinant Mouse EGF R, His Tag (EGR-M5224) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 647 (Accession # Q01279-1).
Predicted N-terminus: Leu 25

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 71.2 kDa. The protein migrates as 86-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20^°C to -70^°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Surface proteome of plasma extracellular vesicles as mechanistic and clinical biomarkers for malaria"
Jung, Møller Jørgensen, Bæk et al
Infection (2023)
(2) "Sub-4 nm mapping of donor-acceptor organic semiconductor nanoparticle composition"
Persson, Laval, Chambon et al
Nanoscale (2023)
(3) "Establishment of Epithelial Inflammatory Injury Model Using Intestinal Organoid Cultures"
Xing, Liang, Yu et al
Stem Cells Int (2023) 2023, 3328655
Showing 1-3 of 3111 papers.