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Recombinant Rhesus Macaque EGFR /ErbB1 Protein, Fc Tag, 100µg  

Recombinant Rhesus Macaque EGFR /ErbB1 Protein, Fc Tag, 100µg

Recombinant Rhesus Macaque EGFR /ErbB1 Protein, produced in human 293 cells (HEK293), Fc Tag (MALS verified)

Synonym: Recombinant Rhesus Macaque protein EGFR, ERBB, ERBB1, HER1, PIG61, mENA

More details

EGR-C5252-100

Availability: within 7 days

300,00 €

Background
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.

Source
Recombinant Rhesus Macaque EGFR /ErbB1 Protein, Fc Tag (EGR-C5252) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P55245).
Predicted N-terminus: Leu 25

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 95.3 kDa. The protein migrates as 116-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Rhesus Macaque EGFR / ErbB1 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) Structural basis of the effect of activating mutations on the EGF receptor
Galdadas, Carlino, Ward et al
Elife (2021) 10
(2) Notch-Jagged signaling complex defined by an interaction mosaic
Zeronian, Klykov, Portell I de Montserrat et al
Proc Natl Acad Sci U S A (2021) 118 (30)
(3) Evaluation of Precise Microwave Ranging Technology for Low Earth Orbit Formation Missions with Beidou Time-Synchronize Receiver
Wang, Wu, Gong et al
Sensors (Basel) (2021) 21 (14)
Showing 1-3 of 2951 papers.
Please refer to product data sheet.